Fig. 2

Melting curves (a), melting peaks (b), normalized melting curves, and temperature shifted difference plot of reference plasmid of P. falciparum, P. ovale wallikeri, P. vivax and P. knowlesi, and P. ovale curtisi. The following melting temperatures were observed: 77.25 °C for P. malariae (grey), 77.69 °C for P. vivax (pink), 78.11°C for P. knowlesi (blue), 78.53 °C for P. ovale wallikeri (brown), 78.73 °C for P. ovale curtisi (green), and 79.01 °C for P. falciparum (red)