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Table 1 Assay melting temperature, specificities, and limits of detection (LOD) with confidence interval (CI)

From: A powerful qPCR-high resolution melting assay with taqman probe in Plasmodium species differentiation

Species

n

Test

Tm (Tm range)

(°C)

Efficiency (%)

Slope

R2

LOD with CI (Copy/µl)

P. malariae

3

HRM

77.25 ± 0.03 (77.20–77.28)

99.85

− 3.3256

0.9974

21.47 (15.97–41.16)

P. vivax

33

HRM

77.69 ± 0.12 (77.50–77.94)

98.78

− 3.3527

0.9985

29.36 (22.61–45.85)

P. knowlesi

5

HRM

78.11 ± 0.06 (78.06–78.22)

99.74

− 3.3281

0.9927

42.47 (31.67–67.57)

P. ovale wallikeri

13

HRM

78.53 ± 0.03 (78.45–78.57)

94.99

− 3.4482

0.9992

33.47 (24.49–57.87)

P. ovale curtisi

5

HRM

78.73 ± 0.05 (78.65–78.79)

97.67

− 3.3791

0.9985

30.26 (22.68–49.81)

P. falciparum

38

HRM

79.01 ± 0.12 (78.80–79.23)

97.95

− 3.3720

0.9991

46.43 (32.50–85.44)

P. falciparum

38

Probe

97.18

− 3.3913

0.9988