Fig. 3

CD8+ T cell and linear B cell Epitopes and amino acids substitution distribution of PfTRAP gene. The full-length TRAP sequences (1–559aa) was referred to the 3D7 isolates (NCBI Gene ID: 814170). Sequences with blue block above is linear B cell epitope; Sequences with orange block above is CD8+ T cell epitope (Detail information of CD8+ T cell epitopes was shown in Additional File 6); Numbers in the color blocks are the corresponding epitope ID of IEDB database. Sequences in gray block is the repeat region. Amino acid in red is for mutant