Skip to main content

Table 1 Primer design information of PfTRAP gene

From: Genetic diversity and natural selection on the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum on Bioko Island, Equatorial Guinea and global comparative analysis

Primer name Primer sequence Production length (bp) Start position End position
TRAP-1-outter-F 5′-GATATCACACCAAATAAATTACAC-3′ 1398 − 152 1247
TRAP-1-outter-R 5′-GATTATCGTGCTTATTTTCGG-3′
TRAP-1-inner-F 5′-GTATGTGCATGCGTACAAG-3′ 1288 − 82 1207
TRAP-1-inner-R 5′-GTTTTCTTCTCGATCGTCT-3′
TRAP-2-outter-F 5′-CACTAAATCCAGAAGAAGGAA-3′ 902 986 + 162
TRAP-2-outter-R 5′-CAGCTTATTCTTTTTTATCCTTAC-3′
TRAP-2-inner-F 5′-GAAAATCCAGAAAATCCACC-3′ 727 1039 + 50
TRAP-2-inner-R 5′-GTTGTTGTGTATTTCACTATATTAC-3′
  1. The Start position number with minus sign (−) indicated the number of nucleotides that before TRAP 5′ terminus. The End position number with plus sign (+) indicated the number of nucleotides that after TRAP 3′ terminus