Table 1 Primer design information of PfTRAP gene
Primer name | Primer sequence | Production length (bp) | Start position | End position |
|---|---|---|---|---|
TRAP-1-outter-F | 5′-GATATCACACCAAATAAATTACAC-3′ | 1398 | − 152 | 1247 |
TRAP-1-outter-R | 5′-GATTATCGTGCTTATTTTCGG-3′ | |||
TRAP-1-inner-F | 5′-GTATGTGCATGCGTACAAG-3′ | 1288 | − 82 | 1207 |
TRAP-1-inner-R | 5′-GTTTTCTTCTCGATCGTCT-3′ | |||
TRAP-2-outter-F | 5′-CACTAAATCCAGAAGAAGGAA-3′ | 902 | 986 | + 162 |
TRAP-2-outter-R | 5′-CAGCTTATTCTTTTTTATCCTTAC-3′ | |||
TRAP-2-inner-F | 5′-GAAAATCCAGAAAATCCACC-3′ | 727 | 1039 | + 50 |
TRAP-2-inner-R | 5′-GTTGTTGTGTATTTCACTATATTAC-3′ |