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Fig. 2 | Malaria Journal

Fig. 2

From: The new Internal Transcribed Spacer 2 diagnostic tool clarifies the taxonomic position and geographic distribution of the North American malaria vector Anopheles punctipennis

Fig. 2

Restriction fragment length polymorphism approach for detecting An. punctipennis. Results of gel electrophoresis of the ITS2 PCR product digested with restriction enzyme Nael is shown for the following species: (1) An. crucians A (644 bp); (2) An. crucians C (387 bp); (3) An. crucians D (476 bp); (4) An. crucians E (378 bp); ; (5) An. quadrimaculatus (470 bp); (6) An. punctipennis, undigested; and (7) An. punctipennis, digested (283 bp and 168 bp). Column M represents a 1 kb DNA ladder (Millapore Sigma, Sigma Aldrich USA). Only the An. punctipennis ITS2 PCR product was cleaved by the enzyme resulting in the appearance of two bands

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