Fig. 2From: The new Internal Transcribed Spacer 2 diagnostic tool clarifies the taxonomic position and geographic distribution of the North American malaria vector Anopheles punctipennisRestriction fragment length polymorphism approach for detecting An. punctipennis. Results of gel electrophoresis of the ITS2 PCR product digested with restriction enzyme Nael is shown for the following species: (1) An. crucians A (644 bp); (2) An. crucians C (387 bp); (3) An. crucians D (476 bp); (4) An. crucians E (378 bp); ; (5) An. quadrimaculatus (470 bp); (6) An. punctipennis, undigested; and (7) An. punctipennis, digested (283 bp and 168 bp). Column M represents a 1 kb DNA ladder (Millapore Sigma, Sigma Aldrich USA). Only the An. punctipennis ITS2 PCR product was cleaved by the enzyme resulting in the appearance of two bandsBack to article page