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Table 2 Predicted sizes of ITS2 PCR products and patterns of restriction fragments in American species of Anopheles based on GenBank data available at [34]

From: The new Internal Transcribed Spacer 2 diagnostic tool clarifies the taxonomic position and geographic distribution of the North American malaria vector Anopheles punctipennis

Species Predicted PCR product size (bp) Predicted RFLP pattern
An. punctipennis 451 283, 168
An. aquasalis 532 356, 176
An. crucians B 1056 731, 145, 93, 87
An. darlingi 592 403, 189
An. neivai 490 275, 215
An. pseudopunctipennis 555 380, 175
An. punctimacula 445 272, 173