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Table 1. 18S rRNA gene qPCR performance characteristics

From: Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

Analytical sensitivity
 Limit of detection (LOD) 2.045 log10 parasites/mL of whole blood
 Lower limit of quantification (LLoQ) 1.50 log10 parasites/mL of whole blood
 Upper limit of quantification (ULoQ) 6.50 log10 parasites/mL of whole blood
Reportable range 1.50 to 6.50 log10 parasites/mL of whole blood
 Precision
  Intra-assay variability Overall standard deviation (SD) = 0.456 Cq units (0.137 log10 parasites/mL)
  Inter-assay variability Overall standard deviation (SD) = 0.604 Cq units (0.182 log10 parasites/mL)
 Accuracy
  EQA program Bias: + 0.019 log10 parasites/mL against nominal parasitaemia values [38]
 Specificity
  Analytical specificity 100%
  Diagnostic specificity 100%