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Fig. 2 | Malaria Journal

Fig. 2

From: Maintaining Plasmodium falciparum gametocyte infectivity during blood collection and transport for mosquito feeding assays in the field

Fig. 2

Infectivity of cultured gametocytes following thermos flask storage. Mosquito infection rate (intensity of oocyst infection) was compared when a gametocyte infected blood meal was fed to mosquitoes immediately (black circles) or stored in a thermos flask for 4 h (red circles). Two storage condition variables were evaluated; (i) the starting water temperature in the thermos flask (37.8 °C, 37 °C or 35.5 °C) and, (ii) the storage temperature of the sealed thermos flask (RT, 32 °C or 42 °C). Graphs show the intensity of infection (oocysts per mosquito) on the left Y axis (circles) and the prevalence of infection (% of mosquitoes infected) on the right Y axis (bars) using the different conditions, (lines indicate the median). A total of 30–60 mosquitoes in 2 or 3 cups were used for each experimental condition and each condition was evaluated 3 to 4 times in independent replicate experiments. Groups were compared by Kruskal–Wallis test comparing the oocyst intensity in each experimental condition to its own control with Dunns multiple comparison test. Statistical comparisons indicated in black show significant reductions, and in blue show significant increases in transmission

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