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Table 1 Effect of gametocyte blood meal storage on infectivity to mosquitoes

From: Maintaining Plasmodium falciparum gametocyte infectivity during blood collection and transport for mosquito feeding assays in the field

Thermos water temperature Thermos storage temperature Total number of mosquitoes evaluated per condition (controls) Number of experiments with a significant reduction in infectivity after 4 h storage
37.8 °C RT 110 (110) 2/3
32 °C 120 (120) 1/3
42 °C 150 (150) 3/4
37 °C RT 180 (178) 0/3
32 °C 140 (140) 1/3
42 °C 180 (180) 2/3
35.5 °C RT 160 (160) 0/3
32 °C 140 (140) 0/3
42 °C 200 (200) 2/4
  1. Mosquito infection rate (intensity of oocyst infection) was compared when a gametocyte infected blood meal was fed to mosquitoes immediately or stored in a thermos flask for 4 h at a range of temperatures. A total of 30–60 mosquitoes in 2 or 3 cups were used for each experimental condition and each experiment was repeated 3 to 4 times. Groups were compared by Kruskal–Wallis comparing each experimental condition to its own control with Dunns multiple comparison test. Graphs showing the full results of the experiments are presented in Fig. 2