Table 1 HRM primers used in this study
Reaction system | Primer name | G6PD variant | Primer sequence (from 5’ to 3’) | Primer concentration (nM) | Amplicon size (bp) | Tm of PCR product (°C) |
|---|---|---|---|---|---|---|
1 | A95G_F | Gaohe | TTCCATCAGTCGGATACACG | 600 | 100 | 81.05 |
A95G_R | (His32Arg) | AGGCATGGAGCAGGCACTTC | 600 | |||
G487A_F | Mahidol | TCCGGGCTCCCAGCAGAA | 400 | 87 | 84.80 | |
G487A_R | (Gly163Ser) | GGTTGGACAGCCGGTCA | 400 | |||
G871A_F | Viangchan | GGCTTTCTCTCAGGTCAAGA | 400 | 66 | 78.32 | |
G871A_R | (Val291Met) | CCCAGGACCACATTGTTGGC | 400 | |||
G1376T_F | Canton | CCTCAGCGACGAGCTCCT | 600 | 99 | 83.65 | |
G1376T_R | (Arg459Leu) | CTGCCATAAATATAGGGGATGG | 600 | |||
2 | G392T_F | Chinese-4 | CATGAATGCCCTCCACCTGGT | 200 | 87 | 85.05 |
G392T_R | (Gly131Val) | TTCTTGGTGACGGCCTCGTA | 200 | |||
C1024T_F | Chinese-5 | CACTTTTGCAGCCGTCGTCT | 400 | 99 | 83.10 | |
C1024T_R | (Leu342Phe) | CACACAGGGCATGCCCAGTT | 400 | |||
C1360T_F | Union | GAGCCAGATGCACTTCGTGT | 200 | 127 | 87.67 | |
C1360T_R | (Arg454Cys) | GAGGGGACATAGTATGGCTT | 200 | |||
G1388A_F | Kaiping | GCTCCGTGAGGCCTGGCA | 400 | 57 | 78.97 | |
G1388A_R | (Arg463His) | TTCTCCAGCTCAATCTGGTGC | 400 |