HRP2 and HRP3 cross-reactivity and implications for HRP2-based RDT use in regions with Plasmodium falciparum hrp2 gene deletions

Table 1 Antigen concentrations for culture parasite dilutions

Parasite density (p/µL)	3D7 (HRP2+/HRP3+)	Dd2 (HRP2−/HRP3+)	HB3 (HRP2+/HRP3−)	3BD5 (HRP2−/HRP3−)
Parasite density (p/µL)	Antigen concentration (ng/mL)
HRP2
100,000	481.26	192.46	376.15	0.00
10,000	344.98	15.25	29.27	0.00
1000	47.86	0.20	3.02	0.00
100	3.25	0.08	0.40	0.00
10	0.45	0.06	0.08	0.00
1	0.17	0.00	0.00	0.00
0.1	0.07	0.00	0.00	0.00
pLDH
100,000	12406.91	4007.06	5379.97	5213.57
10,000	1557.34	474.12	534.33	677.61
1000	146.28	57.74	92.89	72.14
100	33.06	22.19	30.46	24.83
10	0.00	0.00	0.00	0.00
1	0.00	0.00	0.00	0.00
0.1	0.00	0.00	0.00	0.00
Aldolase
100,000	90.76	280.24	223.95	81.21
10,000	20.94	23.35	16.96	11.68
1000	1.78	3.06	1.94	1.09
100	0.36	1.11	0.76	0.41
10	0.00	0.78	0.27	0.10
1	0.00	0.19	0.14	0.00
0.1	0.00	0.00	0.00	0.00

ISSN: 1475-2875