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Table 2 Limit of detection

From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP

Using blood samples Using recombinant plasmid by each target
PvCOX1-LAMP LOD 2.4 parasites/μL 95%CI 1.8–4.8 PvCOX1-LAMP LOD 23.9 copies/μL 95%CI 16.1–65.8
Parasites/μLa: Positive/n° replicates Plasmid (Copies/μL): Positive/n° replicates
214.1 15/15 1684.0 15/15
21.4 15/15 168.4 15/15
2.1 13/15 16.8 13/15
1.1 9/15 8.5 7/15
0.5 0/15 3.4 1/15
Pvr364-LAMP LOD 3.7 parasites/μL 95%CI 2.8–7.5 Pvr364-LAMP LOD 27.3 copies/μL 95%CI 19.1–133.6
3D7 culture (Parasites/μL): Positive/n° replicates Plasmid (Copies/μL): Positive/n° replicates
314.2 15/15 1865.5 15/15
31.4 15/15 186.6 15/15
3.1 13/15 18.7 12/15
1.6 6/15 9.3 4/15
0.8 0/15 1.9 0/15
Pvr47-LAMP LOD 3 parasites/μL 95%CI: 2.1–6.8 Pvr47-LAMP LOD 15.6 copies/μL 95%CI: 11.9–30.5
Parasites/μLa: Positive/n° replicates Plasmid (Copies/μL): Positive/n° replicates
214.1 15/15 1631.6 15/15
21.4 15/15 163.2 15/15
2.1 12/15 16.3 14/15
1.1 5/15 8.2 9/15
0.5 0/15 3.3 0/15
PfEMP1-LAMP LOD 3.3 parasites/μL 95%CI: 2.4–6.7 Pvr47-LAMP LOD 28.9 copies/μL 95%CI: 21.2–86.7
3D7 culture (Parasites/μL): Positive/n° replicates Plasmid (Copies/μL): Positive/n° replicates
314.2 15/15 2076.0 15/15
31.4 15/15 207.6 15/15
3.1 14/15 20.8 12/15
1.6 8/15 10.4 3/15
0.8 1/15 2.1 0/15
  1. aPool of blood samples from infected patients quantified in parasite/µL by real-time PCR