Table 2 Limit of detection
From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP
Using blood samples | Using recombinant plasmid by each target | ||
|---|---|---|---|
PvCOX1-LAMP LOD 2.4 parasites/μL 95%CI 1.8–4.8 | PvCOX1-LAMP LOD 23.9 copies/μL 95%CI 16.1–65.8 | ||
Parasites/μLa: | Positive/n° replicates | Plasmid (Copies/μL): | Positive/n° replicates |
214.1 | 15/15 | 1684.0 | 15/15 |
21.4 | 15/15 | 168.4 | 15/15 |
2.1 | 13/15 | 16.8 | 13/15 |
1.1 | 9/15 | 8.5 | 7/15 |
0.5 | 0/15 | 3.4 | 1/15 |
Pvr364-LAMP LOD 3.7 parasites/μL 95%CI 2.8–7.5 | Pvr364-LAMP LOD 27.3 copies/μL 95%CI 19.1–133.6 | ||
|---|---|---|---|
3D7 culture (Parasites/μL): | Positive/n° replicates | Plasmid (Copies/μL): | Positive/n° replicates |
314.2 | 15/15 | 1865.5 | 15/15 |
31.4 | 15/15 | 186.6 | 15/15 |
3.1 | 13/15 | 18.7 | 12/15 |
1.6 | 6/15 | 9.3 | 4/15 |
0.8 | 0/15 | 1.9 | 0/15 |
Pvr47-LAMP LOD 3 parasites/μL 95%CI: 2.1–6.8 | Pvr47-LAMP LOD 15.6 copies/μL 95%CI: 11.9–30.5 | ||
|---|---|---|---|
Parasites/μLa: | Positive/n° replicates | Plasmid (Copies/μL): | Positive/n° replicates |
214.1 | 15/15 | 1631.6 | 15/15 |
21.4 | 15/15 | 163.2 | 15/15 |
2.1 | 12/15 | 16.3 | 14/15 |
1.1 | 5/15 | 8.2 | 9/15 |
0.5 | 0/15 | 3.3 | 0/15 |
PfEMP1-LAMP LOD 3.3 parasites/μL 95%CI: 2.4–6.7 | Pvr47-LAMP LOD 28.9 copies/μL 95%CI: 21.2–86.7 | ||
|---|---|---|---|
3D7 culture (Parasites/μL): | Positive/n° replicates | Plasmid (Copies/μL): | Positive/n° replicates |
314.2 | 15/15 | 2076.0 | 15/15 |
31.4 | 15/15 | 207.6 | 15/15 |
3.1 | 14/15 | 20.8 | 12/15 |
1.6 | 8/15 | 10.4 | 3/15 |
0.8 | 1/15 | 2.1 | 0/15 |