From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP
Mini Spin Column | Boil & Spin | |||||||
---|---|---|---|---|---|---|---|---|
PvCOX1-LAMP & Pfr364-LAMP | PvCOX1-LAMP & PfEMP1-LAMP | PvCOX1-LAMP & Pfr364-LAMP | PvCOX1-LAMP & PfEMP1-LAMP | |||||
P. vivax and P. falciparum infection detected by microscopy & PCR | 100.0% | 95 CI 85–100% | 100.0% | 95 CI 85–100% | 85.7% | 95 CI 66.4–95.3% | 85.7% | 95 CI 66.4–95.3% |
n = 28 | n = 28 | n = 28 | n = 24 | n = 24 | ||||
P. vivax and P. falciparum submicroscopic infection detected only by PCR | 80.2% | 95 CI 70.8–87.2% | 84.2% | 95 CI 75.3–90.4% | 36.6% | 95 CI 27.4–46.86% | 34.7% | 95 CI 25.64–44.9% |
n = 101 | n = 81 | n = 85 | n = 37 | n = 35 | ||||
Non P. vivax and P. falciparum infection detected by Microscopy & PCR | 97.3% | 95 CI 93.4–99% | 96.7% | 95 CI 92.7–98.7% | 100.0% | 95 CI 97.4–100% | 100.0% | 95 CI 97.4–100% |
n = 183 | n = 178 | n = 177 | n = 183 | n = 183 | ||||
Sensitivitya | 84.5% | 95 CI 78.3–90.7% | 87.6% | 95 CI 81.9–93.3% | 47.3% | 95 CI 38.7–55.9% | 45.7% | 95 CI 37.1–54.3% |
Specificitya | 97.3% | 95CI 94.9–99.6% | 96.7% | 95 CI 94.1–99.3% | 100.0% | 95CI 100–100% | 100.0% | 95CI 100–100% |
Agreement Cohen’s Kappaa | 0.832 | 0.769–0.895 | 0.853 | 0.794- 0.912 | 0.513 | 0.423–0.602 | 0.497 | 0.407–0.587 |