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Table 5 Sensitivity and specificity for P. vivax and P. falciparum detection

From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP

 

Mini Spin Column

Boil & Spin

PvCOX1-LAMP & Pfr364-LAMP

PvCOX1-LAMP & PfEMP1-LAMP

PvCOX1-LAMP & Pfr364-LAMP

PvCOX1-LAMP & PfEMP1-LAMP

P. vivax and P. falciparum infection detected by microscopy & PCR

100.0%

95 CI 85–100%

100.0%

95 CI 85–100%

85.7%

95 CI 66.4–95.3%

85.7%

95 CI 66.4–95.3%

n = 28

n = 28

n = 28

n = 24

n = 24

P. vivax and P. falciparum submicroscopic infection detected only by PCR

80.2%

95 CI 70.8–87.2%

84.2%

95 CI 75.3–90.4%

36.6%

95 CI 27.4–46.86%

34.7%

95 CI 25.64–44.9%

n = 101

n = 81

n = 85

n = 37

n = 35

Non P. vivax and P. falciparum infection detected by Microscopy & PCR

97.3%

95 CI 93.4–99%

96.7%

95 CI 92.7–98.7%

100.0%

95 CI 97.4–100%

100.0%

95 CI 97.4–100%

n = 183

n = 178

n = 177

n = 183

n = 183

Sensitivitya

84.5%

95 CI 78.3–90.7%

87.6%

95 CI 81.9–93.3%

47.3%

95 CI 38.7–55.9%

45.7%

95 CI 37.1–54.3%

Specificitya

97.3%

95CI 94.9–99.6%

96.7%

95 CI 94.1–99.3%

100.0%

95CI 100–100%

100.0%

95CI 100–100%

Agreement Cohen’s Kappaa

0.832

0.769–0.895

0.853

0.794- 0.912

0.513

0.423–0.602

0.497

0.407–0.587

  1. aReference method: real-time PCR described by Mangold et al.[40]