Genetic diversity and genetic relatedness in Plasmodium falciparum parasite population in individuals with uncomplicated malaria based on microsatellite typing in Eastern and Western regions of Uganda, 2019–2020

Table 4 pfhrp2 Amino Acid Repeat types, translated sequences and frequencies (n = 62)

PfHRP2		Total (N = 62)	Region		RDT and LM		p-value
PfHRP2			East	West	RDT−/LM +	RDT+/LM +
Code	Repeat sequences		(N = 40)	(N = 22)	(N = 24)	(N = 38)
1	AHHAHHVAD	2.7 ± 1.4	1–7	0–6	2.9 ± 1.5	2.6 ± 1.3	0.504
2	AHHAHHAAD	11.8 ± 2.2	10–17	8–13	12.1 ± 1.9	11.7 ± 2.4	0.427
3	AHHAHHAAY	1.2 ± 0.7	0–2	0–3	1.1 ± 0.7	1.3 ± 0.7	0.457
4	AHH	0.5 ± 0.8	0–3	0–1	0.3 ± 0.6	0.6 ± 0.9	0.146
5	AHHAHHASD	0.7 ± 0.5	0–2	0–1	0.8 ± 0.5	0.6 ± 0.5	0.221
6	AHHATD	3.0 ± 1.4	1–6	2–5	3.0 ± 1.3	3.0 ± 1.4	1
7	AHHAAD	6.5 ± 2.4	4–11	4–8	6.2 ± 2.1	6.8 ± 2.5	0.317
8	AHHAAY	1.1 ± 0.6	0–2	1–2	1.2 ± 0.8	1.1 ± 0.5	0.85
9	AAY	0.0 ± 0.0	0.0	0.0	0.0 ± 0.0	0.0 ± 0.0	NA
10	AHHAAAHHATD	1.6 ± 0.7	0–3	0–2	1.9 ± 0.7	1.5 ± 0.6	0.018
11	AHN	0.0 ± 0.0	0.0	0.0	0.0 ± 0.0	0.0 ± 0.0	NA
12	AHHAAAHHEAATH	0.0 ± 0.0	0	0	0.0 ± 0.0	0.0 ± 0.0	NA
13	AHHASD	0.0 ± 0.0	0	0	0.0 ± 0.0	0.0 ± 0.0	NA
14	AHHAHHATD	0.3 ± 0.4	0–1	0–1	0.4 ± 0.5	0.2 ± 0.4	0.097
*New		0.0 ± 0.3	0–2	0	0.0 ± 0.0	0.1 ± 0.3	0.324

*New = are sequences observed in Ugandan isolates that had not been reported before. East and West are the Eastern and Western regions of Uganda. Data was given by mean + -SD. P-value was calculated by Student’s t-test or Wilcoxon’s Rank Sum test as appropriate. LM = microscopy

ISSN: 1475-2875