Fig. 2From: Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cyclePlasmodium knowlesi recognizes P. vivax promoter regions not recognized by P. falciparum. Plasmodium falciparum and P. knowlesi parasites were transformed in vitro by spontaneous DNA uptake from RBCs pre-loaded with plasmids pD-pfcam-Luc, pD-pvcam-Luc, pD-pvcrt-Luc, or pD-pvhsp70-Luc. Luciferase activity measurements were obtained after 72 h of parasite cultivation in the plasmid-loaded RBCs and were normalized relative to the activity obtained from parasites transformed with pD-pfcam-Luc. Values represent the mean ± standard error from three independent experimentsBack to article page