Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Table 1 Antimalarial half-maximum inhibitory concentration (IC₅₀) values obtained by SYBR Green and NanoLuc assays

P. knowlesi parasite line	Drug	SYBR Green method			NanoLuc method
P. knowlesi parasite line	Drug	IC₅₀	SEM	N	IC₅₀	SEM	N
H strain (untransfected)	Chloroquine	17.06 nM	1.82^a	2	NA	NA	NA
	WR99210	0.07 nM	0.01	3	NA	NA	NA
	G418	213.85 μM	60.17^a	2	NA	NA	NA
pvcen-pvhsp70-D-NanoLuc	Chloroquine	16.81 nM	1.91	3	16.13 nM	1.54	4
	WR99210	6.59 nM	1.54	3	7.36 nM	2.14	5
	G418	270.80 μM	57.13^a	2	239.63 μM	22.59	3

N Number of independent biological replicates performed, SEM Standard Error of the Mean, NA not applicable
^aStandard Error is presented for the IC₅₀ values obtained using two biological replicates

ISSN: 1475-2875