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Table 1 Metabolomic studies of in vitro red blood cell culture systems originally designed to study P. falciparum intraerythrocytic development

From: Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells

Study Label Date of experimenta RBC infection status RPMI culture medium changeb References
Action Component
1 Pure 1 4/2016 Uninfected None [9]
2 −Hxn 5/2017 Uninfected Reduce to 0.5 μM Hypoxanthine [7]
3  + Mev 3/2018 Uninfected Add 50 μM Mevalonate [8]
4  + Fos 11/2018 Uninfected Add 1.0 μM Fosmidomycin [10]
5 Pure 2 5/2019 Uninfected None This study
6 iRBC 4/2016 Infected None [9]
  1. aAll experiments started within 2–3 days of blood collection. bAll experiments used Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Gaithersburg, MD, USA) supplemented with 20 mM HEPES, 90 µM hypoxanthine, 0.3% sodium bicarbonate, 25 µg/mL gentamicin, 0.5 µM R-lipoic acid, and 0.5% AlbuMAX II (Life Technologies, Carlsbad, CA, USA)
  2.  + Fos fosmidomycin-added RPMI medium, − Hxn hypoxanthine-limited RPMI medium, iRBC parasite-infected red blood cells, + Mev mevalonate-added RPMI medium, Pure 1 pure RPMI medium, Pure 2 pure RPMI medium, RBC red blood cells