Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells

Malaria Journal

Table 1 Metabolomic studies of in vitro red blood cell culture systems originally designed to study P. falciparum intraerythrocytic development

Study	Label	Date of experiment^a	RBC infection status	RPMI culture medium change^b		References
Study	Label	Date of experiment^a	RBC infection status	Action	Component	References
1	Pure 1	4/2016	Uninfected	None	–	[9]
2	−Hxn	5/2017	Uninfected	Reduce to 0.5 μM	Hypoxanthine	[7]
3	+ Mev	3/2018	Uninfected	Add 50 μM	Mevalonate	[8]
4	+ Fos	11/2018	Uninfected	Add 1.0 μM	Fosmidomycin	[10]
5	Pure 2	5/2019	Uninfected	None	–	This study
6	iRBC	4/2016	Infected	None	–	[9]

^aAll experiments started within 2–3 days of blood collection. ^bAll experiments used Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Gaithersburg, MD, USA) supplemented with 20 mM HEPES, 90 µM hypoxanthine, 0.3% sodium bicarbonate, 25 µg/mL gentamicin, 0.5 µM R-lipoic acid, and 0.5% AlbuMAX II (Life Technologies, Carlsbad, CA, USA)
+ Fos fosmidomycin-added RPMI medium, − Hxn hypoxanthine-limited RPMI medium, iRBC parasite-infected red blood cells, + Mev mevalonate-added RPMI medium, Pure 1 pure RPMI medium, Pure 2 pure RPMI medium, RBC red blood cells

ISSN: 1475-2875