Fig. 5

Flow cytometry analysis of splenocyte subsets at 3, 5, 8 dpi. Splenocytes of mice infected with parasites were incubated with the required antibodies according to the manufacture's protocol for antibody dilution, incubation duration, and were analysed in a CytoFLEX flow cytometer. *, ** and *** indicate statistical significance at p < 0.05, p < 0.01 and p < 0.001 compared with the uninfected group, respectively (Data are presented as the mean ± SD, n = 6). a Representative dot plots of splenocytes gated for CD45⁺. Percentages in the right quadrant indicate the frequencies of CD45⁺ cells within total splenocytes. b Representative dot plots of CD3⁺ splenocytes expressing CD45⁺. Percentages in the upper right quadrant indicate the frequencies of CD3⁺ cells within total CD45⁺ cells. c Representative dot plots of CD4⁺ and CD8⁺ gated on CD45⁺CD3⁺ splenocytes. Percentages in the upper left quadrant indicate the frequencies of CD4⁺ cells within total CD45⁺CD3⁺ cells, percentages in the lower right quadrant indicate the frequencies of CD8⁺ cells within total CD45⁺CD3⁺ cells. d Representative dot plots of F4/80⁺ splenocytes. Percentages in the right quadrant indicate the frequencies of F4/80⁺ cells within total splenocyte