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Table 1 PCR primers for the eight Anopheles species

From: Multiplex PCR assay for the identification of eight Anopheles species belonging to the Hyrcanus, Barbirostris and Lindesayi groups

Species Universal forward primer (5′→3′) Reverse primer (5′→3′) Product length (bp)
  ATC GAT GAA GAC CGC AGC TA   
Anopheles sinensis   TAG GGT CAA GGC ATA CAG AAG G 1112
Anopheles koreicus   TAT CGT GGC CCT CGA CAG 925
Anopheles lindesayi   ACC ATC TAC TGC CTG AAC GTG 650
Anopheles kleini   TTT GTT GAT AAC TTG TAT CGT CCA TC 527
Anopheles lesteri   CAG TCT CTT GCA GCC CAT TC 436
Anopheles sineroides   CGC GCA CGC TCA GAT ATT 315
Anopheles belenrae   TGT CCT AGG CGG TTA TCA ACA 260
Anopheles pullus   CGG CGT AGT TTA TTG TGT ATA ACA TC 157