Fig. 2

Transfection of PfMDR1 1246Y over time. Sequence traces for 4 independent transfections are displayed (A–D). Sequencing was conducted 3–4 weeks post-transfection and then weekly during cloning and expansion. The sequences shown are for amino acids 1244 to 1248. Nucleotides highlighted in blue and with a vertical line represent those subject to the T to G substitution that corresponds to a tyrosine (Y, mutant) to aspartic acid (D, wild type) substitution