Fig. 1

Primary sequence alignment of the modelled residues of PfVIT with the plant EgVIT1 template. The target-template alignment used for construction of the PfVIT homology model was generated by HHblits [49]. Conserved, similar, and non-conserved amino acids are highlighted in dark blue, light blue, and white, respectively. Conserved glutamate and methionine residues involved in coordination of substrate metal cation(s) within the cytoplasmic metal binding domain (MBD) are presented in red boxes. The positions of the transmembrane spanning helices (TM1 to TM5) and helices H1 to H3 of the cytoplasmic MBD of the plant VIT1 structure are indicated