Fig. 6

Iron tolerance of ccc1Δ yeast conferred by the functional expression of PfVIT cytoplasmic metal binding domain glutamine mutants. Cultures of ccc1Δ yeast cells that expressed wild type sPfVIT, glutamine mutant sPfVIT or empty pESC-Leu vector were diluted in twofold steps (from OD₆₀₀ 0.2 to 0.0125), spotted onto SC agar plates lacking leucine and supplemented with a no added Fe²⁺ as a control or b 7.5 mM Fe²⁺. The plates were incubated at 30 °C for 3 days. c Quantitative analysis of colony growth (counted as colonies formed per ml of OD1 culture) of ccc1Δ yeast cells that expressed wild type sPfVIT, glutamine mutant sPfVIT or empty pESC-Leu vector on SC-Leu agar plates that contained no added iron. d Colony formation of ccc1Δ yeast cells that expressed wild type sPfVIT, glutamine mutant sPfVIT or empty pESC-Leu vector on SC-Leu agar plates that contained 7.5 mM Fe²⁺. For each yeast transformant, colony formation is quantitated as a percentage of that of the respective control grown on plates with no added iron. Data in c and d are presented as the mean ± s.e.m. of three independent experiments. The data were analysed using one-way analysis of variance (ANOVA) and Dunnett’s multiple comparison tests to determine any statistically significant difference between growth of yeast cells that expressed wild type and mutant sPfVIT; **P < 0.01, ***P < 0.001, ****P < 0.0001