Day -4 | Isolate B-cells from PBMC using CD19 + Dynabeads |
Day -3 | Detatch B-cells from Dynabeads |
Irradiate CD40L + mouse fibroblasts | |
Put B-cells together with irradiated CD40L + cells for activation | |
Day 0 | Put activated B-cells and P. falciparum together in co-culture |
Day 2–9 | Daily monitoring of the co-culture and counting of parasitaemia by microscopy |
Media change with/without addition of cytokines and recomb CD40L on alternate days. Addition of fresh RBC if needed | |
Day 10 | Flow cytometry of B-cells |