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Table 1 Characteristics of the included studies

From: Toll-like receptor 9 and 4 gene polymorphisms in susceptibility and severity of malaria: a meta-analysis of genetic association studies

Author, year [Ref] SNP Country Setting Age group in cases,yr Male % in cases Cases Controls Genotyping method Consistent with HWE
Mockenhaupt, 2006 [20] TLR4-A299G, T399I
TLR9 T-1237C, T1486C
Ghana H Children 46.6 290 UM: 290
Healthy: 290
allele-specific PCR and PCR-RT Yes
Leoratti, 2008 [21] TLR4 D299G
TLR9 T1237C, T1486C
Brazil H md 29 86 230 74 PCR–RFLP Yes
Sam-Agudu, 2010 [22] TLR4-A299G, T399I
TLR9-T1486C, T1237C
Uganda H 6.2 (2.4) 63.1 Cases (SM): 65 Controls (UM: 52 PCR and LDR-FMA Yes
Zakeri, 2011
[23]
TLR4 D299G, T399I
TLR9
T1486C, T1237C
Iran HCs md 28 65.6 P. falciparum infected: 320 Healthy control: 320 PCR–RFLP Yes
Esposito, 2012 [24] TLR4 A299G (rs4986790)
TLR9 T1486C (rs187084), T1237C (rs5743836)
Burundi H Children 57.5 602 337 Taqman Yes
Munde, 2012
[25]
TLR9 T1237C Kenya H Children 47.8 SM: 138 UM: 163 TaqMan Yes
Sawian, 2012
[26]
TLR4 A299G
TLR9
T1237C, T1486C
India H md: 26.2 (R2-58)   SM: 136 Controls (UM: 53 PCR–RFLP No
Kar, 2015
[27]
TLR4 D299G
TLR9 T1237C, T1486C
India H SM: 38.0 (25.0–53.7)
UM: 38.0 (24.2–55.0)
58% 200 200 PCR–RFLP Yes (D299G)
No (T1237C and T1486C)
Iwalokun, 2015 [28] TLR4 A299G, T399I Nigeria H Children 62.1 182 97 PCR–RFLP Yes
Costa, 2017 [29] TLR4 A299G (rs4986790), T399I (rs4986791)
TLR9-T1237C (rs187084), T1486C (rs5743836)
Brazil   37 (19 ± 53) 61.5 Pv-325 Healthy group: 274 PCR–RFLP Yes (A299G, T399I, T1237C)
No (T1486C)
Rani, 2018 [30] TLR4 A299G Pakistan H 22.7 ± 13.6 53.5 228 (SM: 89, UM: 139) 226 Allele-specific PCR No
  1. H hospital, HC Primary Health Centre, HWE HardyWeinberg equilibrium, LDR-FMA ligase detection reaction-fluorescent microsphere assay, md median, PCR–RFLP polymerase chain reaction-restriction fragment length polymorphism fragment length, PCR-RT real-time PCR, R range, SM severe malaria/complicated malaria/cerebral malaria, SNP single nucleotide polymorphism, TaqMan TaqMan allelic discrimination/SNP assay, UM uncomplicated malaria/mild malaria, Yes/No controls are/are not consistent with HWE