Fig. 1

Representative melting curves of P. falciparum and P. vivax. The x-axis shows the temperature (°C). The y-axis shows the negative derivative of fluorescence (RFU) with respect to temperature (T). The Plasmodium species is indicated above each melting curve diagram. Red curves correspond to the Texas Red labelled probe, purple curves to the Cy5 labelled probe, and brown curves to the Cy5.5 labelled probe. For the sake of clarity, the melting temperature thresholds were manually set at zero