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Table 3 Melting temperatures of the Texas Red, Cy5 and Cy5.5 Labelled probes for 11 Plasmodium species/strains

From: Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans

Plasmodium species

Melting temperature (°C)

Texas Red labelled probe

Melting temperature (°C)

Cy5 labelled probe

Melting temperature (°C)

Cy5.5 labelled probe

P. falciparum

42.4

54.4

None

P. vivax

49.2

40.4

51.6

P. malariae

39.6

33.6

None

P. ovale wallikeri

49.6

48.4

None

P. ovale curtisi

53.2

48.4

None

P. knowlesi LT48

49.2

40.8

62.8

P. knowlesi ATCC 30158

56.4

40.8

62.8

P. knowlesi ATCC 30153

56.4

40.8

52.4

P. cynomolgi ATCC 30149

56.0

40.4

57.6

P. cynomolgi KJ569866.1

56.4

40.8

54.8

P. cynomolgi KJ569868.1

50.8

40.8

58.8

  1. Differences in probe melting temperatures enable distinction between the 11 Plasmodium species/strains