Skip to main content

Table 3 Melting temperatures of the Texas Red, Cy5 and Cy5.5 Labelled probes for 11 Plasmodium species/strains

From: Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans

Plasmodium species Melting temperature (°C)
Texas Red labelled probe
Melting temperature (°C)
Cy5 labelled probe
Melting temperature (°C)
Cy5.5 labelled probe
P. falciparum 42.4 54.4 None
P. vivax 49.2 40.4 51.6
P. malariae 39.6 33.6 None
P. ovale wallikeri 49.6 48.4 None
P. ovale curtisi 53.2 48.4 None
P. knowlesi LT48 49.2 40.8 62.8
P. knowlesi ATCC 30158 56.4 40.8 62.8
P. knowlesi ATCC 30153 56.4 40.8 52.4
P. cynomolgi ATCC 30149 56.0 40.4 57.6
P. cynomolgi KJ569866.1 56.4 40.8 54.8
P. cynomolgi KJ569868.1 50.8 40.8 58.8
  1. Differences in probe melting temperatures enable distinction between the 11 Plasmodium species/strains