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Fig. 1 | Malaria Journal

Fig. 1

From: Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Fig. 1

Schematic diagram of the workflow for predicting species composition ratio. The COI fragment were amplified and sequenced from pooled gDNA specimens. Based on the sequencing chromatograms, the nucleotide signals of three COI loci were quantified to estimate the composition ratios of Anopheles sineroides, Anopheles pullus, and Anopheles lesteri. Next, the ITS2 gene fragments were selectively amplified and sequenced for Anopheles sinensis, Anopheles kleini, and Anopheles belenrae. The nucleotide signals of two ITS2 loci were quantified from the sequence chromatograms and the composition ratios of An. sinensis, An. kleini, and An. belenrae were deduced

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Malaria Journal

ISSN: 1475-2875

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