Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Table. 2 Designed primer sets used for predicting relative species composition ratios

Gene	Primer name	Sequence (5′–3′)	Size (bp)
COI	An_COI-F	CTTTAAGTATTCTAATTCGAGCTG	594
COI	An_COI-R	TAAAATWGGRTCTCCTCCTCC	594
ITS2	Anskb_ITS2-F	CAGACAAGTAGAAAGGGCTGT	234^a/235^b/238^c
ITS2	Anskb_ITS2-R	ACAAATCTGGGTAGTGTTCTCT	234^a/235^b/238^c

ISSN: 1475-2875