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Table. 3 Linear regression analysisa results of species-specific nucleotide position of COI gene

From: Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Locus Species distinction Nucleotide Nb R2 Sest c y = f(x)
mC.260A An. sineroides A 6 0.995 1.49 1.039x + 1.446
mC.443T An. sineroides T 6 0.993 1.71 0.9831x + 1.910
mC.122C An. sineroidesAn. pullus C 14 0.997 1.78 0.9942x + 2.369
mC.387C An. pullus C 11 0.995 2.49 1.051x + 0.289
mC.582C An. pullus C 11 0.985 4.26 1.027x + 1.412
mC.525C skb C 11 0.998 1.51 1.008x + 0.0612
mC.527T skb T 11 0.998 1.63 0.9903x – 1.457
mC.590C skb C 11 0.991 3.24 0.9821x + 2.635
  1. aLinear regression analyses for 8 nucleotide positions were done by GraphPad Prism. The selected markers and corresponding equations for species distinction are underlined
  2. bThe number of x values used for regression analysis
  3. cStandard error of estimate