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Table. 3 Linear regression analysisa results of species-specific nucleotide position of COI gene

From: Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Locus

Species distinction

Nucleotide

Nb

R2

Sest c

y = f(x)

mC.260A

An. sineroides

A

6

0.995

1.49

1.039x + 1.446

mC.443T

An. sineroides

T

6

0.993

1.71

0.9831x + 1.910

mC.122C

An. sineroidesAn. pullus

C

14

0.997

1.78

0.9942x + 2.369

mC.387C

An. pullus

C

11

0.995

2.49

1.051x + 0.289

mC.582C

An. pullus

C

11

0.985

4.26

1.027x + 1.412

mC.525C

skb

C

11

0.998

1.51

1.008x + 0.0612

mC.527T

skb

T

11

0.998

1.63

0.9903x – 1.457

mC.590C

skb

C

11

0.991

3.24

0.9821x + 2.635

  1. aLinear regression analyses for 8 nucleotide positions were done by GraphPad Prism. The selected markers and corresponding equations for species distinction are underlined
  2. bThe number of x values used for regression analysis
  3. cStandard error of estimate