Prediction of species composition ratios in pooled specimens of the Anopheles Hyrcanus group using quantitative sequencing

Table. 5 Evaluation of the accuracy of the predicted composition ratios from the pooled gDNA and pooled mosquito specimens

Specimen condition	Data set	Number of data points	r^a	p	R²	MAE^b	Min-max error
Pooled gDNA specimen	All	45	0.984	< 0.0001	0.968	3.87	0.10–12.2
	COI	21	0.977	< 0.0001	0.955	4.45	0.10–12.2
	ITS2	30	0.982	< 0.0001	0.965	4.02	0.12–12.2
	An. pullus	15	0.992	< 0.0001	0.984	3.57	0.10–7.34
	An. kleini	15	0.991	< 0.0001	0.982	3.28	0.15–10.8
	An. sinensis	15	0.983	< 0.0001	0.966	4.76	0.12–12.2
Pooled mosquito specimen	All	36	0.985	< 0.0001	0.971	2.90	0.06–13.2
	COI	24	0.987	< 0.0001	0.974	3.59	0.19–13.2
	ITS2	18	0.984	< 0.0001	0.968	2.21	0.06–12.8
	An. pullus	6	0.985	0.0002	0.970	4.36	0.17–10.9
	An. kleini	6	0.985	0.0002	0.971	2.83	0.06–12.8
	An. sinensis	6	0.990	< 0.0001	0.980	3.15	0.16–12.1
	An. belenrae	6	0.997	< 0.0001	0.994	0.66	0.07–1.56
	An. lesteri^c	6	N/A	N/A	N/A	3.85	1.51–8.13
	An. sineroides	6	0.984	0.0002	0.968	1.09	0.19–1.45

^aPearson correlation coefficient
^bMean absolute error
^cStatistical analysis of An. lesteri was not possible since all the actual value was zero

ISSN: 1475-2875