Fig. 1

Sankey diagram showing the diagnostic strategy for methods to diagnose malaria within and outside office hours. The diagnostic strategy for the laboratory diagnosis of malaria within office hours (panel A) and outside office hours (panel B) are shown. Since outside office hours many laboratories use a different strategy depending on the result of the screening assay, panel B is split in two graphs showing the workflow in case of positive (panel B1) or negative test result of the screening assay (panel B2). MD: missing data; NA: not applicable; Ext: externally performed; LAMP: loop mediated isothermal amplification; ICT: immunochromatographic test; rtPCR: real-time polymerase chain reaction; QBC: Quantitative Buffy Coat; im: immediately; nm: next morning; *if histidine-rich protein 2 (HRP-2) antigen is positive in ICT