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Table 3 Functional prediction of mutation sites

From: Population genetic analysis of the Plasmodium falciparum erythrocyte binding antigen-175 (EBA-175) gene in Equatorial Guinea

  Mutation sites ΔΔG Poly-Phen-2 PROVEAN
Score (HumDiv)a Sensitivity Specificity Classificationb Score Predictionc
F1 domain A34T 4.725 0.999 0.14 0.99 Probably damaging − 1.151 Neutral
K49E 0.273 0.022 0.95 0.80 Benign − 1.356 Neutral
E97K − 0.043 0.000 1.00 0.00 Benign 0.031 Neutral
I98K 1.842 0.002 0.95 0.30 Benign 1.265 Neutral
K102E − 0.358 0.004 0.97 0.59 Benign − 0.756 Neutral
K109E 0.075 0.810 0.84 0.93 Probably damaging 0.781 Neutral
E120K − 0.524 0.025 0.95 0.81 Benign − 0.569 Neutral
D159Y − 1.942 0.075 0.93 0.84 Benign 0.377 Neutral
K211N 0.586 0.000 1.00 0.00 Benign 0.504 Neutral
P213S 0.693 0.000 1.00 0.00 Benign 0.689 Neutral
E226K − 0.685 0.000 1.00 0.00 Benign 0.479 Neutral
N227K 0.474 0.033 0.95 0.82 Benign − 0.443 Neutral
K228M − 0.164 0.002 0.99 0.30 Benign − 0.258 Neutral
N238S 0.012 0.000 1.00 0.00 Benign − 1.585 Neutral
  D278Y − 0.434 1.000 0.00 1.00 Probably damaging − 2.728 Deleterious
F2 domain K301N 1.225 0.592 0.87 0.91 Probably damaging 0.388 Neutral
K304I − 0.007 0.000 1.00 0.00 Benign − 0.861 Neutral
L305V 1.551 0.825 0.84 0.93 Probably damaging − 0.204 Neutral
D329N 0.021 0.777 0.85 0.92 Probably damaging − 0.69 Neutral
N400K − 0.660 0.001 0.99 0.15 Benign − 0.002 Neutral
V402A − 0.047 0.006 0.97 0.75 Benign − 0.272 Neutral
Q407K 0.032 0.001 0.99 0.15 Benign 0.539 Neutral
Q407E − 1.657 0.000 1.00 0.00 Benign 0.149 Neutral
E415A 0.020 0.198 0.92 0.88 Benign − 1.077 Neutral
  1. aHumDiv is the preferred model for evaluating rare alleles, dense mapping of regions identified by genome-wide association studies, and analysis of natural selection
  2. bHumDivA >  = 0.953, probably damaging; 0.953 > HumDivB >  = 0.432, possibly damaging; 0.432 > HumDivC >  = 0.0024, benign
  3. cThe default threshold is − 2.5; that is, variants with a score equal to or below − 2.5 are considered "deleterious" and variants with a score above − 2.5 are considered "neutral."