Population genetic analysis of the Plasmodium falciparum erythrocyte binding antigen-175 (EBA-175) gene in Equatorial Guinea

Table 3 Functional prediction of mutation sites

	Mutation sites	ΔΔG	Poly-Phen-2				PROVEAN
	Mutation sites	ΔΔG	Score (HumDiv)^a	Sensitivity	Specificity	Classification^b	Score	Prediction^c
F1 domain	A34T	4.725	0.999	0.14	0.99	Probably damaging	− 1.151	Neutral
	K49E	0.273	0.022	0.95	0.80	Benign	− 1.356	Neutral
	E97K	− 0.043	0.000	1.00	0.00	Benign	0.031	Neutral
	I98K	1.842	0.002	0.95	0.30	Benign	1.265	Neutral
	K102E	− 0.358	0.004	0.97	0.59	Benign	− 0.756	Neutral
	K109E	0.075	0.810	0.84	0.93	Probably damaging	0.781	Neutral
	E120K	− 0.524	0.025	0.95	0.81	Benign	− 0.569	Neutral
	D159Y	− 1.942	0.075	0.93	0.84	Benign	0.377	Neutral
	K211N	0.586	0.000	1.00	0.00	Benign	0.504	Neutral
	P213S	0.693	0.000	1.00	0.00	Benign	0.689	Neutral
	E226K	− 0.685	0.000	1.00	0.00	Benign	0.479	Neutral
	N227K	0.474	0.033	0.95	0.82	Benign	− 0.443	Neutral
	K228M	− 0.164	0.002	0.99	0.30	Benign	− 0.258	Neutral
	N238S	0.012	0.000	1.00	0.00	Benign	− 1.585	Neutral
	D278Y	− 0.434	1.000	0.00	1.00	Probably damaging	− 2.728	Deleterious
F2 domain	K301N	1.225	0.592	0.87	0.91	Probably damaging	0.388	Neutral
	K304I	− 0.007	0.000	1.00	0.00	Benign	− 0.861	Neutral
	L305V	1.551	0.825	0.84	0.93	Probably damaging	− 0.204	Neutral
	D329N	0.021	0.777	0.85	0.92	Probably damaging	− 0.69	Neutral
	N400K	− 0.660	0.001	0.99	0.15	Benign	− 0.002	Neutral
	V402A	− 0.047	0.006	0.97	0.75	Benign	− 0.272	Neutral
	Q407K	0.032	0.001	0.99	0.15	Benign	0.539	Neutral
	Q407E	− 1.657	0.000	1.00	0.00	Benign	0.149	Neutral
	E415A	0.020	0.198	0.92	0.88	Benign	− 1.077	Neutral

^aHumDiv is the preferred model for evaluating rare alleles, dense mapping of regions identified by genome-wide association studies, and analysis of natural selection
^bHumDivA > = 0.953, probably damaging; 0.953 > HumDivB > = 0.432, possibly damaging; 0.432 > HumDivC > = 0.0024, benign
^cThe default threshold is − 2.5; that is, variants with a score equal to or below − 2.5 are considered "deleterious" and variants with a score above − 2.5 are considered "neutral."

ISSN: 1475-2875