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Table 2 Assay precision for DBS samples. Each result generated as copies/mL DBS lysate, converted log copies/mL blood to calculate within-run and within-lab percent coefficient of variation (% CV) and 95% confidence interval (95% CI)

From: Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies

qRT-PCR

Control

N (days)

Mean log10 copies/mL blood

Within-run % CV (95% CI)

Within-lab %CV (95% CI)

Pf

High positive

20 (10)

8.66

0.97% (0.68–1.71%)

3.32% (2.52–4.85%)

Low positive

20 (10)

6.69

2.73% (1.91–4.79%)

4.07% (3.10–5.95%)

Pan

High positive

20 (10)

8.62

1.10% (0.77–1.93%)

3.99% (3.03–5.83%)

Low positive

20 (10)

6.32

3.29% (2.30–5.77%)

5.61% (4.26–8.19%)

  1. Pf P. falciparum, Pan pan-Plasmodium