Fig. 2

Screening Plasmodium falciparum patient isolates. Flow diagram of screening and selection procedure. Vials from liquid nitrogen were thawed and isolates were tested for adaptation to the automated shaker culture system (dashed line) [16]. After adaptation vials were frozen to expand the number of aliquots. Isolates were genotyped, transferred to automated tipper culture system [18] and tested for their ability to produce viable gametocytes that can initiate transmission to mosquito vector. Successful isolates were cloned by limiting dilution, cryopreserved and tested again after thawing (solid line). The minimum time from adaptation to successful cloning and establishment of a new clonal isolate was estimated 30 weeks