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Fig. 3 | Malaria Journal

Fig. 3

From: A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes

Fig. 3

Selection and exclusion of Plasmodium falciparum isolates. Flow diagram of in- and excluded isolates in this study. Out of 121 isolates 21 were selected on their origin and tested according to the process presented in Fig. 2. Out of this selection 12 were excluded for cloning: 11 isolates showed no transmission and 1 produced no gametocytes. The remaining 9 isolates were cloned by limiting dilution and tested subsequently. After cloning, 1 isolate was excluded for showing no transmission (NF153.E8) and 8 isolates were found eligible for use in future transmission research. Eligible isolates were categorized on transmission intensity into two groups with mean infection intensities above or below 5 oocysts per mosquito

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