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Table 2 Pairwise beta and alpha diversity comparisons of laboratory colonized F1 An. albimanus microbiota from different collection sites. Bray-Curtis diversity comparison showed significant differences in larval internal and cuticle surface microbiota between collection sites. In contrast, only adult cuticle surface but not internal microbiota were significantly different across collection sites. These comparisons were conducted using PERMANOVA (999 permutations) tests, with Benjamini-Hochberg FDR correction (q-value). Significance was determined at q < 0.05. n = No. of pools processed, and each pool comprised three individual mosquitoes.

From: Comprehensive characterization of internal and cuticle surface microbiota of laboratory-reared F1 Anopheles albimanus originating from different sites

 

Group 1

Group 2

Internal

Cuticle surface

pseudo-F

p-value

q-value

pseudo-F

p-value

q-value

Adults

LasCruces1 (n = 9)

LasCruces3 (n = 15)

2.21

0.088

0.161

5.80

0.001

0.003

 

LasCruces1 (n = 9)

LasCruces4 (n = 21)

1.92

0.107

0.161

4.97

0.004

0.005

 

LasCruces3 (n = 15)

LasCruces4 (n = 21)

0.85

0.443

0.443

3.07

0.005

0.005

Larvae

ElTerreroa (n = 15)

LasCruces3 (n = 14)

9.81

0.001

0.001

4.48

0.001

0.001

 

ElTerreroa (n = 15)

LasCruces4 (n = 15)

7.98

0.001

0.001

11.43

0.001

0.001

 

LasCruces3 (n = 14)

LasCruces4 (n = 15)

10.88

0.001

0.001

11.37

0.001

0.001

  1. aTwo pools were exlcuded from the analysis of cuticle surface microbiota following rarefaction