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Table 3 Prevalence of polymorphisms from pre-treatment samples observed during a therapeutic efficacy study in Madagascar, stratified by eventual outcome

From: Efficacy of artesunate-amodiaquine and artemether-lumefantrine for uncomplicated Plasmodium falciparum malaria in Madagascar, 2018

  Overall
N = 85
ACPR
N = 65
ETF
N = 1
Recrudescence
N = 4
Reinfection
N = 14
pfk13a n = 83 n = 65 n = 1 n = 3 n = 14
 Wild type (no mutations detected) 83 (100%) 65 (100%) 1 (100%) 3 (100%) 14 (100%)
pfmdr1 N86Y
 Successfully amplified n = 81 n = 62 n = 1 n = 4 n = 14
 N 64 (79%) 48 (78%) 1 (100%) 3 (75%) 12 (86%)
 N/Y 2 (3%) 2 (3%) 0 (0%) 0 (0%) 0 (0%)
 Y 15 (19%) 12 (19%) 0 (0%) 1 (25%) 2 (14%)
Y184F
 Successfully amplified n = 82 n = 63 n = 1 n = 4 n = 14
 Y 34 (41%) 23 (36%) 1 (100%) 1 (25%) 9 (64%)
 Y/F 7 (9) 6 (10%) 0 (0%) 0 (0%) 1 (7%)
 F 41 (50%) 34 (54%) 0 (0%) 3 (75%) 4 (29%)
S1034C
 Successfully amplified n = 77 n = 59 n = 1 n = 4 n = 13
 S 77 (100%) 59 (100%) 1 (100%) 4 (100%) 13 (100%)
N1042D
 Successfully amplified n = 77 n = 59 n = 1 n = 4 n = 13
 N 77 (100%) 59 (100%) 1 (100%) 4 (100%) 13 (100%)
D1246Y
 Successfully amplified n = 77 n = 59 n = 1 n = 4 n = 13
 D 73 (95%) 57 (97%) 1 (100%) 3 (75%) 12 (92%)
 D/Y 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%)
 Y 4 (5%) 2 (3%) 0 (0%) 1 (25%) 1 (8%)
pfmdr1 haplotypesb
 Amplified for all 3 codons (86, 184, 1246) n = 74 n = 56 n = 1 n = 4 n = 13
 NFD 37 (50%) 28 (50%) 1 (100%) 3 (75%) 5 (27%)
 NYD 30 (41%) 23 (41%) 0 (0%) 0 (0%) 7 (38%)
 YFD 6 (8%) 5 (9%) 0 (0%) 1 (25%) 0 (0%)
 YYD 8 (11%) 6 (11%) 0 (0%) 0 (0%) 2 (11%)
 YYY 1 (1%) 0 (0%) 0 (0%) 0 (0%) 1 (5%)
 YFY 1 (1%) 1 (1%) 0 (0%) 0 (0%) 0 (0%)
 NFY 2 (3%) 1 (1%) 0 (0%) 0 (0%) 1 (5%)
pfcrt haplotypes
 Amplified for all 5 codons (72—76) n = 82 n = 63 n = 1 n = 4 n = 14
 CVMNK 82 (100%) 63 (100%) 1 (100%) 4 (100%) 14 (100%)
  1. ACPR adequate clinical and parasitological response, ETF early treatment failure
  2. aInvestigated SNPs: F446I, N456Y, M476I, Y493H, R539T, I543T, P553L, R561H, C580Y
  3. bmixed infections included in the numerator for each haplotype; therefore, column totals may sum to > 100%