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Table 1 Primers used in this study for the molecular identification of Anopheles complex/group members in village and farm/forest sites in Ia DReh commune, Krong Pa district, and Ia KDam commune, Ia Pa district, Gia Lai Province, Vietnam

From: Entomological survey in two communes with residual malaria transmission in Gia Lai Province in the central highlands of Vietnam

Species Primer name Sequence (5ʹ-3ʹ) PCR size References
Universal forward primer ITS2A 5ʹ TGT GAA CTG CAG GAC ACA T 3ʹ   Garros et al. [26]
An. minimus MIA 5ʹ CCC GTG CGA CTT GAC GA 3ʹ 310-bp Garros et al. [26]
An. harrisoni MIC 5ʹ GTT CAT TCA GCA ACA TCA GT 3ʹ 180-bp Garros et al. [26]
An. aconitus ACO 5ʹ ACA GCG TGT ACG TCC AGT 3ʹ 200-bp Garros et al. [26]
An. varuna VAR 5ʹ TTG ACC ACT TTC GAC GCA 3ʹ 260-bp Garros et al. [26]
An. pampanai PAM 5ʹ TGT ACA TCG GCC GGG GTA 3ʹ 90-bp Garros et al. [26]
An. dirus (A) FA2 5ʹ TCG GGT TCT ATA ATA TTC GCT 3ʹ 120-bp Huong et al. [27]
RA2 5ʹ GAC CTA GTG TTT GGG AAG GT3ʹ
An. cracens FB2 5ʹ GCT TCA AGA CCA AAA CCA TCA3ʹ 75-bp Huong et al. [27]
RB2 5ʹ GAA TTT ACA ACT TTT GAC CTG G3ʹ
An. scanloni (C) FC3 5ʹ ATT CTG TGC CAA AAT TGT ACC T3ʹ 60-bp Huong et al. [27]
RC3 5ʹ TTG TCC GAA ACT GGC TTC T 3ʹ
An. baimaii FD1 5ʹ AGG GCA CAA AAG TTA TTA ACT T 3ʹ 172-bp Huong et al. [27]
RD1 5ʹ GTG AAG AGC GAA TAT TGT AGC 3ʹ
Universal forward primer 5.8F 5ʹ ATC ACT CGG CTC GTG GAT CG 3ʹ   Walton et al. [20]
An. maculatus MAC 5ʹ GAC GGT CAG TCT GGT AAA GT 3ʹ 180-bp Walton et al. [20]
An. pseudowillmori PSEU 5ʹ GCC CCC GGG TGT CAA ACA G 3ʹ 203-bp Walton et al. [20]
An. sawadwongporni SAW 5ʹ ACG GTC CCG CAT CAG GTG C 3ʹ 242-bp Walton et al. [20]
An. rampae (form k) K 5ʹ TTC ATC GCT CGC CCT TAC AA 3ʹ 301-bp Walton et al. [20]
An. dravidicus DRAV 5ʹ GCC TAC TTT GAG CGA GAC CA 3ʹ 477-bp Walton et al. [20]