Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate

Table 1 Amount of nHRP2 obtained from HB3 culture supernatant from two experiments

Experiment	Sample	Imidazole (mM)	HRP2 conc (ng/mL)	Volume obtained (mL)	Total nHRP2 (µg)
1	Culture supernatant		40.88	3662	149.711
1	Column flow-through		0.89	3662	3.262
1	Wash 1	10	0.07	45	0.003
1	Wash 2	10	0.03	47	0.001
1	Wash 3	20	0.04	50	0.002
1	Wash 4	20	0.04	47	0.002
1	Wash 5	50	0.28	9	0.003
1	Wash 6	50	2.22	9	0.020
1	Elution 1	500	10,542.21	10	105.422
1	Elution 2	500	3548.24	10	35.482
1	Elution 3	1000	1331.44	6.5	8.654
1				TOTAL µg eluted	149.6
1				% Recovery	99.9
2	Culture supernatant		9.61	480	4.612
2	Column flow-through		0.11	480	0.054
2	Wash 1	10	0.05	11.5	0.001
2	Wash 2	10	0.01	12	0.000
2	Wash 3	10	0.00	11	0.000
2	Wash 4	10	0.00	12.5	0.000
2	Wash 5	10	0.00	12	0.000
2	Wash 6	10	0.00	12	0.000
2	Wash 7	20	0.01	7.5	0.000
2	Wash 8	20	0.01	13	0.000
2	Wash 9	20	0.01	9	0.000
2	Elution 1	500	3311.53	1.5	4.967
2	Elution 2	500	2926.64	1.5	4.390
2	Elution 3	500	1826.29	1.5	2.739
2	Elution 4	500	705.83	1.5	1.059
2	Elution 5	500	214.17	2	0.428
2				TOTAL µg eluted	13.6
2				% Recovery	295.7

ISSN: 1475-2875