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Table 2 Amount of nHRP2 obtained from HB3 infected RBCs from two experiments

From: Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate

Experiment

Sample

Imidazole (mM)

HRP2 conc (ng/mL)

Volume obtained (mL)

Total nHRP2 (µg)

1

Lysed RBC supernatant

 

87.58

1800.0

157.652

1

Column flow-through

 

0.42

1800.0

0.753

1

Wash 1

10

0.04

35.0

0.002

1

Wash 2

10

0.03

40.0

0.001

1

Wash 3

10

0.03

42.0

0.001

1

Wash 4

10

0.02

42.0

0.001

1

Wash 5

20

0.02

32.0

0.001

1

Wash 6

20

0.01

40.0

0.001

1

Wash 7

20

0.01

40.0

0.001

1

Wash 8

50

0.03

45.0

0.001

1

Wash 9

50

0.05

45.0

0.002

1

Wash 10

50

0.03

48.0

0.001

1

Elution 1

500

6934.61

8.0

55.477

1

Elution 2

500

5400.09

7.0

37.801

1

Elution 3

500

2730.68

5.5

15.019

1

Elution 4

500

1537.56

9.5

14.607

1

   

TOTAL µg eluted

122.9

1

   

% Recovery

78.0

2

Lysed RBC supernatant

 

65.20

500.0

32.598

2

Column flow through

 

1.69

500.0

0.846

2

Wash 1

10

0.84

20.0

0.017

2

Wash 2

10

0.19

9.5

0.002

2

Wash 3

10

0.03

11.0

0.000

2

Wash 4

10

0.01

11.5

0.000

2

Wash 5

10

0.03

9.0

0.000

2

Wash 6

10

0.01

11.5

0.000

2

Wash 7

10

0.01

12.0

0.000

2

Wash 8

20

0.01

9.0

0.000

2

Wash 9

20

0.01

11.0

0.000

2

Wash 10

20

0.00

11.5

0.000

2

Elution 1

500

365.03

2.0

0.730

2

Elution 2

500

1426.91

2.0

2.854

2

Elution 3

500

881.73

2.0

1.763

2

Elution 4

500

304.55

3.0

0.914

2

   

TOTAL µg eluted

6.3

2

   

% Recovery

19.3