Experiment | Sample | Imidazole (mM) | HRP2 conc (ng/mL) | Volume obtained (mL) | Total nHRP2 (µg) |
---|---|---|---|---|---|
1 | Lysed RBC supernatant | Â | 87.58 | 1800.0 | 157.652 |
1 | Column flow-through | Â | 0.42 | 1800.0 | 0.753 |
1 | Wash 1 | 10 | 0.04 | 35.0 | 0.002 |
1 | Wash 2 | 10 | 0.03 | 40.0 | 0.001 |
1 | Wash 3 | 10 | 0.03 | 42.0 | 0.001 |
1 | Wash 4 | 10 | 0.02 | 42.0 | 0.001 |
1 | Wash 5 | 20 | 0.02 | 32.0 | 0.001 |
1 | Wash 6 | 20 | 0.01 | 40.0 | 0.001 |
1 | Wash 7 | 20 | 0.01 | 40.0 | 0.001 |
1 | Wash 8 | 50 | 0.03 | 45.0 | 0.001 |
1 | Wash 9 | 50 | 0.05 | 45.0 | 0.002 |
1 | Wash 10 | 50 | 0.03 | 48.0 | 0.001 |
1 | Elution 1 | 500 | 6934.61 | 8.0 | 55.477 |
1 | Elution 2 | 500 | 5400.09 | 7.0 | 37.801 |
1 | Elution 3 | 500 | 2730.68 | 5.5 | 15.019 |
1 | Elution 4 | 500 | 1537.56 | 9.5 | 14.607 |
1 |  |  |  | TOTAL µg eluted | 122.9 |
1 | Â | Â | Â | % Recovery | 78.0 |
2 | Lysed RBC supernatant | Â | 65.20 | 500.0 | 32.598 |
2 | Column flow through | Â | 1.69 | 500.0 | 0.846 |
2 | Wash 1 | 10 | 0.84 | 20.0 | 0.017 |
2 | Wash 2 | 10 | 0.19 | 9.5 | 0.002 |
2 | Wash 3 | 10 | 0.03 | 11.0 | 0.000 |
2 | Wash 4 | 10 | 0.01 | 11.5 | 0.000 |
2 | Wash 5 | 10 | 0.03 | 9.0 | 0.000 |
2 | Wash 6 | 10 | 0.01 | 11.5 | 0.000 |
2 | Wash 7 | 10 | 0.01 | 12.0 | 0.000 |
2 | Wash 8 | 20 | 0.01 | 9.0 | 0.000 |
2 | Wash 9 | 20 | 0.01 | 11.0 | 0.000 |
2 | Wash 10 | 20 | 0.00 | 11.5 | 0.000 |
2 | Elution 1 | 500 | 365.03 | 2.0 | 0.730 |
2 | Elution 2 | 500 | 1426.91 | 2.0 | 2.854 |
2 | Elution 3 | 500 | 881.73 | 2.0 | 1.763 |
2 | Elution 4 | 500 | 304.55 | 3.0 | 0.914 |
2 |  |  |  | TOTAL µg eluted | 6.3 |
2 | Â | Â | Â | % Recovery | 19.3 |