Experiment | Sample | Imidazole (mM) | HRP2 conc (ng/mL) | Volume obtained (mL) | Total nHRP2 (µg) |
---|---|---|---|---|---|
1 | Parasite pellet lysate | Â | 3.21 | 8.7 | 0.028 |
1 | Flow-through | Â | 0.36 | 50.0 | 0.018 |
1 | Wash 1 | 10 | 0.12 | 15.0 | 0.002 |
1 | Wash 2 | 10 | 0.08 | 10.0 | 0.001 |
1 | Wash 3 | 10 | 0.02 | 10.0 | 0.000 |
1 | Wash 4 | 20 | 0.03 | 5.0 | 0.000 |
1 | Wash 5 | 20 | 0.02 | 4.0 | 0.000 |
1 | Wash 6 | 20 | 0.11 | 5.0 | 0.001 |
1 | Wash 7 | 50 | 0.14 | 4.5 | 0.001 |
1 | Wash 8 | 50 | 0.12 | 2.5 | 0.000 |
1 | Wash 9 | 50 | 0.05 | 4.0 | 0.000 |
1 | Elution 1 | 500 | 84.46 | 1.5 | 0.127 |
1 | Elution 2 | 500 | 70.03 | 1.5 | 0.105 |
1 | Elution 3 | 500 | 31.80 | 1.5 | 0.048 |
1 | Elution 4 | 1000 | 19.03 | 4.5 | 0.086 |
1 |  |  |  | TOTAL µg eluted | 0.37 |
1 | Â | Â | Â | % Recovery | 1306.33 |
2 | Elution 1 | 500 | 1651.23 | 1.5 | 2.477 |
2 | Elution 2 | 500 | 722.49 | 1.5 | 1.084 |
2 | Elution 3 | 500 | 187.24 | 1.5 | 0.281 |
2 | Elution 4 | 500 | 89.64 | 4.5 | 0.403 |
2 |  |  |  | TOTAL µg eluted | 4.2 |