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Table 3 Amount of nHRP2 obtained from HB3 parasite pellet from two experiments

From: Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate

Experiment

Sample

Imidazole (mM)

HRP2 conc (ng/mL)

Volume obtained (mL)

Total nHRP2 (µg)

1

Parasite pellet lysate

 

3.21

8.7

0.028

1

Flow-through

 

0.36

50.0

0.018

1

Wash 1

10

0.12

15.0

0.002

1

Wash 2

10

0.08

10.0

0.001

1

Wash 3

10

0.02

10.0

0.000

1

Wash 4

20

0.03

5.0

0.000

1

Wash 5

20

0.02

4.0

0.000

1

Wash 6

20

0.11

5.0

0.001

1

Wash 7

50

0.14

4.5

0.001

1

Wash 8

50

0.12

2.5

0.000

1

Wash 9

50

0.05

4.0

0.000

1

Elution 1

500

84.46

1.5

0.127

1

Elution 2

500

70.03

1.5

0.105

1

Elution 3

500

31.80

1.5

0.048

1

Elution 4

1000

19.03

4.5

0.086

1

   

TOTAL µg eluted

0.37

1

   

% Recovery

1306.33

2

Elution 1

500

1651.23

1.5

2.477

2

Elution 2

500

722.49

1.5

1.084

2

Elution 3

500

187.24

1.5

0.281

2

Elution 4

500

89.64

4.5

0.403

2

   

TOTAL µg eluted

4.2