Table 4 Band intensity of Carestart Pf HRP2 and HRP2/pLDH RDTs following incubation with varying concentrations of nHRP2, rHRP2, and Plasmodium falciparum culture samples

nHRP2 (derived from HB3 supernatant)

2060 ng/mL

3

4

4

4

206 ng/mL

4

3

4

3

20.6 ng/mL

4

2

4

2

2.06 ng/mL

4

0

4

0

0.206 ng/mL

4

0

4

0

0.0206 ng/mL

N/A

N/A

4

0

rHRP2

2060 ng/mL

4

4

4

4

206 ng/mL

4

4

4

4

20.6 ng/mL

4

4

4

3

2.06 ng/mL

4

2

4

2

0.206 ng/mL

4

0

4

0

0.0206 ng/mL

N/A

N/A

4

0

Pf 3D7 culture

1000 p/µL

N/A

N/A

4

4

500 p/µL

4

4

4

4

200 p/µL

4

4

4

3

100 p/µL

4

3

4

2

50 p/µL

4

2

4

2

25 p/µL

4

2

4

1

12.5 p/µL

4

1

4

0

6.25 p/µL

4

0

4

0

US06 F

Nigeria XII

Culture

1000 p/µL

N/A

N/A

4

4

500 p/µL

4

4

4

4

200 p/µL

4

4

4

3

100 p/µL

4

3

4

2

50 p/µL

4

2

4

1

25 p/µL

4

2

4

1

12.5 p/µL

4

1

4

0

6.25 p/µL

4

0

4

0