Table 1 Oligonucleotide probes used for the detection of avian haemosporidian parasites by chromogenic in situ hybridization
Probe | Sequence | Work concentration | Target | References |
|---|---|---|---|---|
Plasmo18S_1 | 5′-CTTAAACTTCCTTGTGTTAGACACACAAT-3′ | 1 ng/100 µl | Plasmodium spp. | Present study |
Haemo18S_1 | 5′-GCTAACCGTAGTTATAGTCGCCATCTC-3′ | 1 ng/100 µl | Haemoproteus (Parahaemoproteus) spp. | Himmel et al. [56] |
Leuco18S_1 | 5′-TAGGACTCCCCACTTGTCTTTTTCTTGA-3′ | 1 ng/100 µl | Leucocytozoon spp. | Himmel et al. [56] |
Prel18S | 5′-ACCATTTAACACGTATCCGATAAAGCATTACC-3′ | 10 ng/100 µl | Plasmodium relictum (SGS1) | Present study |
Pelo18S | 5′-CAACTGTTACATTGGGACGCCTTT-3′ | 10 ng/100 µl | Plasmodium elongatum (GRW06) | Present study |