Extracellular vesicles in malaria: an agglomeration of two decades of research

Malaria Journal

Table 1 Factors affecting inter-study comparability of human field studies of plasma EVs in malaria

Plasmodium species	Sample size	EV isolation method	EV analysis technique	Plasma EVs analysed	References
P. vivax	37	Medium speed centrifugation (14,000×g)	FC	LEVs, MEVs, REVs, PEVs, EEVs	[17]
P. falciparum	250	Medium speed centrifugation (1500×g)^a	FC	EEVs	[32]
P. falciparum	146	Medium speed centrifugation (13,000×g)^a	FC	LEVs, MEVs, REVs, PEVs, EEVs	[33]
P. falciparum	128	High speed ultracentrifugation (100,000×g)	FC	REVs, PEVs, EEVs	[34]
P. falciparum	128	High speed ultracentrifugation (100,000×g)^a	FC	REVs, PEVs, EEVs	[35]
P. falciparum, P. vivax, P. malariae	36	Medium speed centrifugation (13,000×g)	FC	REVs	[36]
P. falciparum	434	Medium speed centrifugation (15,000×g) for mEVs; High speed ultracentrifugation (100,000×g) for sEVs	FC NTA TEM WBA	Total plasma EVs	[42]

FC flow cytometry, NTA Nanoparticle Tracking Analysis, TEM Transmission Electron Microscopy, WBA Western Blot Analysis; g- relative centrifugal force^a Indicates that the centrifugation speed was not clearly stated in the journal article

ISSN: 1475-2875