Fig. 6

Low erythrocytic BgA-expression levels affects rosette size when exposed to rosette-disruptive agents. Relative rosette size of FCR3S1.2 parasites propagated in RBCs from 30 donors and thereafter exposed to three conditions; A, B untreated, C, D heparin 50 µg/mL and E, F anti-IT4(var60) PfEMP1- DBL1ɑ treated (250 µg/mL). Correlation between A-antigen and rosette size was assessed using Spearman’s rank correlation coefficient for each condition (right panels). Pairwise comparison of BgO and all non-O Bgs performed using unpaired t-test with Holm adjustment of P-values (* P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001). All box plots display median values (middle line) and the lower and upper quartile values form the box, with whiskers extending to one and a half times the interquartile range