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Table 1 Sequence of primers used in nested PCR for the amplification of the pfcrt and pfmdr1 genes

From: Assessment of Plasmodium falciparum anti-malarial drug resistance markers in pfcrt and pfmdr1 genes in isolates from Honduras and Nicaragua, 2018–2021

Gene

Primer

Sequence 5′- 3′

Size bp

References

pfcrt

AL6821

AGCAAAAATGACGAGCGTTATAG

559

[11]

AL6822

ATTGGTAGGTGGAATAGATTCTC

AL5631

TTTTTCCCTTGTCGACCTTAAC

264

AL5632

AGGAATAAACAATAAAGAACATAATCATAC

pfmdr1 SNPs 86, 184

MDR1-1F

TTAAATGTTTACCTGCACAACATAGAAAATT

612

[17]

MDR1-1R

CTCCACAATAACTTGCAACAGTTCTTA

MDR1-2F

TGTATGTGCTGTATTATCAGGA

526

MDR1-2R

CTCTTCTATAATGGACATGGTA

pfmdr1 SNPs 1034, 1046

1042-A

GTCGAAAAGACTATGAAACGTAGA

711

[17]

1042-C

CTCAAATGATAATTTTGCAT

1042-B

GATCCAAGTTTTTTAATACA

1042-C

CTCAAATGATAATTTTGCAT

pfmdr1 SNPs 1246

1246-A

GTGGAAAATCAACTTTTATGA

500

[17]

1246-B

TTAGGTTCTCTTAATAATGCT

1246-C

GACTTGAAAAATGATCACATT

412

1246-D

GTCCACCTGATATGCTTTT