Fig. 2

Nitric oxide produced by inducible nitric oxide synthase (iNOS) is associated with microvesicular fatty infiltration. Immunized female wild-type (WT) and iNOS-knockout (KO) mice were infected intravenously with 1 × 10⁴ erythrocytes infected with P. berghei NK65. A Course of parasitaemia. Closed and opened symbols indicate WT and iNOS-KO mice, respectively. Circles and triangles indicate pregnant and non-pregnant mice, respectively. Results are shown as the means ± standard deviation of three mice. The experiment was performed in triplicate with similar results. B–G Histological examination. Liver tissue staining with haematoxylin and eosin (B–D) and Sudan IV (E–G). Livers were obtained from mice on day 17 post-mating. B, E Immunized non-pregnant WT mice infected with P. berghei NK65. C, F Immunized pregnant WT mice infected with P. berghei NK65. D, G Immunized pregnant iNOS-KO mice infected with P. berghei NK65. Scale bar indicates 100 μm. Data are representative of six independent experiments