Table 2 SNPs detected in pfdhfr and pfdhps genes over the years

1999 (N = 60)

51 (58%)

88.64–99.08

56 (93.3%)

84.07–97.38

56 (93.3%)

88.64–99.08

30 (50%)

37.74–62.24

4 (6.6%)

2.62–15.93

3 (5%)

1.71–13-70

2001 (N = 102)

95 (93.1%)

86.51–96.64

88 (86.3%)

78.27–91.64

83 (81.4%)

72.73–87.74

62 (60.7%)

51.08–69.70

0

0–3.63

0

0–3.63

2004/5 (N = 262)

255 (93.3%)

94.59–98.70

259 (98.8%)

96.69–99.61

257 (98.1%)

95.61–99.18

158 (60.3%)

54.27–66.04

0

0–3.63

0

0–3.63

2006 (N = 158)

150 (94.9%)

90.33–97.41

151 (95.5%)

91.14–97.84

158 (100%)

97.63–100

44 (27.8%)

21.45–35.3

4 (2.5%)

0.99–6.33

0

0–2.37

2011 (N = 163)

159 (97.5%)

93.86–99.04

159 (97.5%)

93.86–99.04

163 (100%)

97.7–100

136 (93.4%)

76.97–88.36

9 (5.5%)

2.93–10.16

3 (1.8%)

0.63–5.27

2013 (N = 170)

168 (98.8%)

95.81–99.68

167 (98.2%)

94.94–99.4

168 (98.8%)

95.81–99.68

149 (87.6%)

81.85–91.78

26 (15.3%)

10.66–21.47

1 (0.58%)

0.10–3.26

2016 (N = 169)

169 (100%)

97.78–100

149 (88.2%)

82.43–92.21

168 (98.8%)

95.81–99.68

156 (92.3%)

87.29–95.45

23 (13.6%)

9.24–19.59

5 (3%)

1.27–6.74

2019 (N = 139)

135 (97.1%)

92.83–98.88

131 (94.2%)

89.05–97.06

139 (100%)

97.31–100

130 (93.5%)

88.15–96.56

51 (36.6%)

29.14–44.96

2 (1.4%)

0.4–5.09

p-value

4.9e−7

7e−

0

0

0

0.0053