Temporal evolution of the resistance genotypes of Plasmodium falciparum in isolates from Equatorial Guinea during 20 years (1999 to 2019)

Table 5 SNPs detected in the pfk13 gene by sequencing

Year (N)	Sample ID	No codon	Wild allele		Mutant allele		Mutation	Type	Freq
Year (N)	Sample ID	No codon	Sequence (nt)	AA	Sequence (nt)	AA	Mutation	Type	Freq
2001 N = 102	136-H	612	gaA	Glu	gaG	Glu	E612E	S	0.98%
2004–5 N = 262	HQ-019	569	gCa	Ala	gTa	Val	A569V	NS	0.76%
2004–5 N = 262	HFA-106	645	aaC	Asn	aaT	Asn	N645N	S	0.76%
2006 N = 158	101_9_3	663	Cta	Leu	Tta	Leu	L663L	S	0.6%
2013 N = 170	210.01.02	469	tgC	Cys	tgT	Cys	C469C	S	1.76%
	110.01.01	510	gTg	Val	gCg	Ala	V510A	NS
	203.01.02	578	Gct	Ala	Tct	Ser	A578S	NS
2019 N = 139	2974	532	tGt	Cys	tAt	Tyr	C532Y	NS	5.03%
	2974	638	Gga	Gly	Aga	Arg	G638R	NS
	3235	469	tgC	Cys	tgT	Cys	C469C	S
	3502	544	Ggg	Gly	Agg	Arg	G544R	NS
	3756	469	tgC	Cys	tgT	Cys	C469C	S
	3812	668	Gag	Glu	Aag	Lys	E668L	NS
	3819	464	Gat	Asp	Aat	Asn	D3819N	NS

SNPs detected in the pfk13 gene after sequencing and their study. The type of mutation detected, synonymous or non-synonymous is indicated
ID: identification; AA: amino acid, nt: nucleotide; Freq.: frequency. A capital letter in the codon sequence indicates the muted nucleotide; S: Synonymous; NS: Non-Synonymous. The nucleotide that changes with the mutation is shown in capital letters and in bold

ISSN: 1475-2875