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Table 4 Loss of infected erythrocytes as a percentage of total erythrocyte loss, in participants inoculated with Pf3D7, PfK13 and P. vivax

From: Haematological response in experimental human Plasmodium falciparum and Plasmodium vivax malaria

Pf all (n = 267)

Median (IQR)

Pf 3D7 (n = 254)

Median (IQR)

Pf K13 (n = 15)

Median (IQR)

P. vivax (n = 46)

Median (IQR)

P-value

(3D7 vs K13) d

P-value

(Pf all vs P. vivax) d

Baseline RCC (× 1012/L)

5.0

(4.7–5.8)

5.0

(4.7–5.8)

5.1

(4.6–5.2)

5.0

(4.7–5.2)

0.58

0.57

Nadir RCC (× 1012/L)

4.5

(4.2–4.8)

4.5

(4.2–4.8)

4.4

(3.8–4.6)

4.4

(4.0–4.8)

0.07

0.31

Total RCC Loss (× 1012/L)

0.5

(0.3–0.7)

0.5

(0.3–0.6)

0.7

(0.6–0.8)

0.5

(0.4–0.6)

0.0005

0.57

Malaria-attributable RCC loss (× 1012/L) a

0.21

(0.04–0.36)

0.20

(0.04–0.33)

0.34

(0.12–0.50)

0.21

(0.04–0.33)

0.06

0.87

Malaria-attributable RCC loss/total RCC loss (%) b

42

(13–51)

40

(13–55)

49

(20–63)

42

(13–55)

0.29

0.46

Loss of pRBC/total malaria-attributable RBC loss (%) c

0.015

(0.006–0.060)

0.014

(0.005–0.039)

0.128

(0.068–0.616)

0.022

(0.008–0.082)

 < 0.0001

0.19

  1. n number of participants; IQR interquartile range
  2. aCalculated by subtracting the estimated phlebotomy-related RBC loss from the total RBC loss. For this calculation, the phlebotomy-related RBC loss was estimated by multiplying the participant’s baseline RCC (× 1012/L) by the estimated total phlebotomy blood volume (0.19L) from day of inoculation to the median day of haemoglobin nadir; bThe percentage contribution of the malaria-attributable erythrocytes losses from the total erythrocyte losses was determined by dividing the malaria-attributable losses by the erythrocyte losses and multiplying by 100 to generate a percentage; cThe loss of pRBCs was equal to the TPBPre, which was determined using the AUC of the 18S qPCR data from day 4 until the time of treatment. For P. falciparum, the TPBPre incorporated an adjustment to account for parasite sequestration, assumed to be an assumed approximately 25% at any given timepoint. This calculation assumed that each erythrocyte was singly infected, and that parasite replication did not continue following treatment; dMann–Whitney test of significance, not adjusted for multiple comparisons